Non-enzymic antioxidants
Many of the diseases are the consequences of free radical damage. The free radicals are nullified by the antioxidants like ascorbic acid, riboflavin, tocopherol, chlorophyll, total carotenoids and flavonoids. The levels of various known non-enzymic antioxidants in Ashshifa-1000 are presented in Table III.
Table-III
NON-ENZYMIC ANTIOXIDANTS IN ASHSHIFA-1000

The above table reveals that the non-enzymic antioxidants, are present in very low amounts.
The results of this investigation suggest that the enzymic antioxidants that might have been present in the fresh herbs used in this formulation are lost during the preparation of the herbal powder in the dry form. It contains only a little amount of the non-enzymic antioxidants.
In vitro inhibition of lipid peroxidation :
Recently, free radical induced lipid peroxidation has been reported to be associated with various deleterious effects including tissue damage and necrosis. The effect of various extracts of Ashshifa-1000 on lipid peroxidation was studied. The results obtained are presented in Table -V.
Table-V
Extent of inhibition of in vitro lipid peroxidation by Ashshifa-1000

The maximum inhibition of lipid peroxidation (LPO) was noted in the ethanol and methanol extracts and the least extent of inhibition was observed in the chloroform extract. The inhibition of in vitro LPO can be attributed to the presence of antioxidants like ascorbic acid, riboflavin, tocopherol and flavonoids.
This study reveals that the maximum inhibition of LPO by the methanol and alcoholic extracts might be due to the tocopherol and flavonoid contents.